Combining Combinatorial Chemistry and Affinity Chromatography

Preparation of Plasminogen by Affinity Chromatography

Background: Plasminogen is one of the compounds derived from human plasma. Activation of plasminogen produces plasmin. Plasmin is able to lyse fibrinogen, fibrin, and some other human plasma proteins. The aim of the present work was to study the separation of human plasminogen by affinity chromatography using gel lysine Sepharose. Materials and Methods: Normal human plasma was used as the...

Combinatorial chemistry

Combinatorial chemistry, which did not even exist fifteen years ago, is a new subfield of chemistry that is starting to provide insights into molecular interactions, biochemical catalysis and drug development. Instead of finding and testing naturally existing compounds for useful activity, the basic aim of combinatorial chemistry studies is to create vast numbers of new, synthetic compounds and...

Combinatorial chemistry.

Concepts of Combinatorial Chemistry and Combinatorial Technologies

Combinatorial Chemistry and Combinatorial Technology (CC/CT) are a new interdisciplinary field joining computerassisted combinatorial chemistry with automated parallel synthesis of chemical ìlibrariesî followed by automated screening. The main purpose of computer assisted combinatorial chemistry is to generate thousands structurally diverse compounds as libraries, maximising their diversity, wh...

Affinity chromatography in dynamic combinatorial libraries: one-pot amplification and isolation of a strongly binding receptor.

We report the one-pot amplification and isolation of a nanomolar receptor in a multibuilding block aqueous dynamic combinatorial library using a polymer-bound template. By appropriate choice of a poly(N,N-dimethylacrylamide)-based support, nonselective ion-exchange type behaviour between the oppositely charged cationic guest and polyanionic hosts was overcome, such that the selective molecular ...